Base Thermal Cycler
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DNA Engine / Dyad Disciple cycler
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Sample capacity
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96-well PCR plate 96 x 0.2 ml tubes
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Sample volume
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10–100 µl (20 µl recommended)
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Dimensions (W x D x H)
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23 x 23 x 18 cm (excluding thermal cycler)
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Weight
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6.8 kg (excluding thermal cycler)
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Fluorescence excitation range
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Channel 1: 450–490 nm
Channel 2: 500–535 nm
Channel 3: 555–585 nm
Channel 4: 620–650 nm
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Fluorescence detection range
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Channel 1: 515–530 nm
Channel 2: 560–580 nm
Channel 3: 610–650 nm
Channel 4: 675–730 nm
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Linear dynamic range of starting copy number
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Up to 10 orders of magnitude
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Detection limit of template starting copy number
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Down to single initial template copy detection
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Gradient specifications
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Gradient range
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30–105°C
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Temperature differential range
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1°C–24°C
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Gradient accuracy
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±0.3°C of programmed target at end columns within 30 sec after gradient step timer starts, NIST traceable
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Temperature uniformity
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±0.4°C, in column, well-to-well, within 30 sec of reaching target temperature
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Gradient calculator accuracy
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±0.4°C of actual well temperature
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Note: Specifications subject to change without notice.
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Minimum Computer Specifications
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Operating system
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Windows XP Professional
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Processor speed
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1.5 GHz
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Storage capacity
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20 GB hard drive
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Communications port
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USB 2.0 high-speed
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Display
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1,024 x 768 pixel minimum screen resolution, 256 colors
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Memory
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512 MB RAM
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Note: Specifications subject to change without notice.
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